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. 2014 Jun 9;197(4):1365–1376. doi: 10.1534/genetics.114.166587

Table 1. Primers used in this study.

Primer name Forward primer Backward primer Notes
Mom1-1 5′-gtaaggtggctccgtggtaa-3′ 5′-catacccaatgccctttttg-3′ Promoter region
Mom1-5′-UTR-1 5′-cccagtcaggagaggtttca-3′ 5′-ggactcattccccagaattg-3′ 5′-UTR region
Mom1-5′-UTR-2 5′-atcggcttgcaaagagaatg-3′ 5′-ctggaaaccactgggacact-3′ 5′-UTR region
Mom1-2 5′-ggcagttggaattcaggaaa-3′ 5′-ttgagcctgaaaggaaatgg-3′
Mom1-3 5′-tcaccaccctttaccaggtc-3′ 5′-acctcggctggctggaaac-3′
Mom1-4 5′-gtaaggccaccccagttctc-3′ 5′-atcttttggccacactctgc-3′
Mom1-5 5′-cctaaaacagggcacacaca-3′ 5′-agtggctgaggatgaccttg-3′
Mom1-6 5′-gccctctgcagtgtatgaaa-3′ 5′tccaagttatgagaacacacacg-3′
Mom1-7 5′-aggccctcacaagtaaagca-3′ 5′-cctggtttttgatggctctc-3′ 3′-UTR region
Mom-2* 5′-accatctctccagcaccaag-3′ 5′-ggcaaaatgagacttaaatgctt-3′ Flanks exon 3 of Atp5a
Mom-5* 5′-tgttggaacgtgtttttgga-3′ 5′-aatcgcatagatacactgtctgag-3′
Mom-7* 5′-aacccaggactgcttccttt-3′ 5′-ttagaaggcaggagcagagg-3′
Primers for screening for Pla2g2a promoter polymorphisms
Pla2g2aF 5′-tgattttgaaacctctcctga-3′ Common primer
Pla2g2aSR 5′-acttcacaaaagcttctgtaac -3′ Wild-type- specific primer
Pla2g2aRR 5′-tcgtatccctgaatgtcttca Polymorphism- specific primer
Primers for cloning Pla2g2a promoter and 5′-UTR regions
Pla2g2a Prom 5′-ggccgcggtaccgtgttctgcctcagctccat- 3′ 5′-gcggcgcctaggtttcctgaattccaactgccc tt-3′
Pla2g2a 5′-UTR 5′-ggccgccctaggaacaagacaaggccttgaacaa-3 5′-gcggcgccatgggctgtcagctctgtgaaggaca-3′
Primers for cloning Pla2g2a coding exons and intervening introns
Pla2 W 5′-ggccgcccatggatgaaggtcctcctgctgctag-3′ 5′-gcggcgtctagattgtctgatgaattgctttacttg-3′
Primers for QRT-PCR for Pla2g2a mRNA
Pla2g2a 5′-tacaagcgcctggagaaaag-3′ 5′-ggccttatcgcactgacaca-3′
Mouse Hprt 5′-tgctcgagatgtcatgaagg-3′ 5′-tatgtcccccgttgactgat-3′ Mouse Hprt
Amp 5′-ggtcctccgatcgttgtcag-3′ 5′-cggtcgccgcatacactatt-3′ Vector Amp
Human HPRT 5′-tgacactggcaaaacaatgca-3′ 5′-ggtccttttcaccagcaagct-3′
Human GAPDH 5′-ccatcactgccacccagaag-3′ 5′-agcttcccgttcagctcagg-3′ DNA sequences