Mom1-1 |
5′-gtaaggtggctccgtggtaa-3′ |
5′-catacccaatgccctttttg-3′ |
Promoter region |
Mom1-5′-UTR-1 |
5′-cccagtcaggagaggtttca-3′ |
5′-ggactcattccccagaattg-3′ |
5′-UTR region |
Mom1-5′-UTR-2 |
5′-atcggcttgcaaagagaatg-3′ |
5′-ctggaaaccactgggacact-3′ |
5′-UTR region |
Mom1-2 |
5′-ggcagttggaattcaggaaa-3′ |
5′-ttgagcctgaaaggaaatgg-3′ |
|
Mom1-3 |
5′-tcaccaccctttaccaggtc-3′ |
5′-acctcggctggctggaaac-3′ |
|
Mom1-4 |
5′-gtaaggccaccccagttctc-3′ |
5′-atcttttggccacactctgc-3′ |
|
Mom1-5 |
5′-cctaaaacagggcacacaca-3′ |
5′-agtggctgaggatgaccttg-3′ |
|
Mom1-6 |
5′-gccctctgcagtgtatgaaa-3′ |
5′tccaagttatgagaacacacacg-3′ |
|
Mom1-7 |
5′-aggccctcacaagtaaagca-3′ |
5′-cctggtttttgatggctctc-3′ |
3′-UTR region |
Mom-2* |
5′-accatctctccagcaccaag-3′ |
5′-ggcaaaatgagacttaaatgctt-3′ |
Flanks exon 3 of Atp5a
|
Mom-5* |
5′-tgttggaacgtgtttttgga-3′ |
5′-aatcgcatagatacactgtctgag-3′ |
|
Mom-7* |
5′-aacccaggactgcttccttt-3′ |
5′-ttagaaggcaggagcagagg-3′ |
|
Primers for screening for Pla2g2a promoter polymorphisms |
Pla2g2aF |
5′-tgattttgaaacctctcctga-3′ |
Common primer |
Pla2g2aSR |
5′-acttcacaaaagcttctgtaac -3′ |
Wild-type- specific primer |
Pla2g2aRR |
5′-tcgtatccctgaatgtcttca |
Polymorphism- specific primer |
Primers for cloning Pla2g2a promoter and 5′-UTR regions |
Pla2g2a Prom |
5′-ggccgcggtaccgtgttctgcctcagctccat- 3′ |
5′-gcggcgcctaggtttcctgaattccaactgccc tt-3′ |
|
Pla2g2a 5′-UTR |
5′-ggccgccctaggaacaagacaaggccttgaacaa-3 |
5′-gcggcgccatgggctgtcagctctgtgaaggaca-3′ |
|
Primers for cloning Pla2g2a coding exons and intervening introns |
Pla2 W |
5′-ggccgcccatggatgaaggtcctcctgctgctag-3′ |
5′-gcggcgtctagattgtctgatgaattgctttacttg-3′ |
|
Primers for QRT-PCR for Pla2g2a mRNA |
Pla2g2a |
5′-tacaagcgcctggagaaaag-3′ |
5′-ggccttatcgcactgacaca-3′ |
|
Mouse Hprt
|
5′-tgctcgagatgtcatgaagg-3′ |
5′-tatgtcccccgttgactgat-3′ |
Mouse Hprt
|
Amp |
5′-ggtcctccgatcgttgtcag-3′ |
5′-cggtcgccgcatacactatt-3′ |
Vector Amp
|
Human HPRT
|
5′-tgacactggcaaaacaatgca-3′ |
5′-ggtccttttcaccagcaagct-3′ |
|
Human GAPDH
|
5′-ccatcactgccacccagaag-3′ |
5′-agcttcccgttcagctcagg-3′ |
DNA sequences |