Figure 4.

Lack of toxic effect of 11a on macrophages. BmMs in ultralow binding tissue culture plates were rested in RPMI complete medium for 24 h before culturing with fresh medium or medium containing 11a (5 μg/mL) or ES-62 (2 μg/mL). After 18 h, the macrophages were stimulated with medium containing 100 ng/mL LPS or 10 ng/mL BLP or 0.01 μM CpG for an additional 24 h before staining with 7-AAD to assess their viability. The samples were analyzed by flow cytometry, and the data are presented as density plots with frequency of 7-AAD positive (dead) cells indicated in the gates. The results shown are from a single experiment representative of two independent experiments.