Fig. 3.
Lymphoproliferation suppression assays were set up and ND (n=3) or HAM/TSP (n=2) CD4+CD25+ T cells were added at different ratios of Treg:Teff = 0.25:1 (A), 0.5:1 (B) and 1:1 (C). The % suppressions of Treg were normalized to Teff proliferation without Treg. (D) Correlation between CD4+CD25+ TSDR % demethylation and suppressive capacity as determined in lymphoproliferation suppression assays at Treg:Teff ratio of 1:1.