Table 1.
HIV neutralization by P16 and PG9 in a pseudovirus reporter gene assay and with PBMC
| Virus | Coreceptor usage |
Antibody | Pseudovirus (luciferase) assay |
PBMC assay | ||
|---|---|---|---|---|---|---|
| IC50 (µg/ml) |
IC90 (µg/ml) |
IC50 (µg/ml) |
IC90 (µg/ml) |
|||
| HIVJR-CSF | R5 | PG16 | 0.001 | 0.012 | 0.049 | 0.249 |
| PG9 | 0.003 | 0.025 | NDc | ND | ||
| HIVJD | R5X4 | PG16 | 0.008 | 0.490 | 0.105 | 2.3 |
| PG9 | 0.074 | 2.3 | ND | ND | ||
| HIVPD (N160K) | X4 | PG16 | >50 | >50 | 2.3 | >10 |
| PG9 | >50 | >50 | ND | ND | ||
| HIVEW(N160K) | X4 | PG16 | >50 | >50 | >10 | >10 |
| PG9 | >50 | >50 | ND | ND | ||
| HIVF | X4 | PG16 | >50 | >50 | >10 | >10 |
| PG9 | >50 | >50 | ND | ND | ||
| HIVJW | R5 | PG16 | 0.12 | >50 | >10 | >10 |
| PG9 | 11 | >50 | ND | ND | ||
| HIVGV | X4 | PG16 | >50 | >50 | >10 | >10 |
| PG9 | >50 | >50 | ND | ND | ||
| HIVNL4-3 | X4 | PG16 | 0.23 | >50 | 0.093 | 0.714 |
| PG9 | 8.9 | >50 | ND | ND | ||
| HIV92/RW/008(clade A) | R5 | PG16 | 0.002a | NRb | 0.046 | 0.520 |
| PG9 | 0.01a | NR | ND | ND | ||
| HIV98/IN/022(clade C) a | R5 | PG16 | 0.003a | NR | 0.071 | 0.443 |
| PG9 | 0.006a | NR | ND | ND | ||
a
Data from Walker et al. (2009). Number of replicates not specified.
b
Not reported.
c
Not determined.
IC50 and IC90 values represent the average of two separate assays for both pseudovirus and PBMC assays.