FIG 2.

Basophil cell-surface expression of CD63 during mammalian meat challenge of patient FC-09 with IgE to alpha-gal (A) and control subjects (B and C). Whole blood was collected at baseline and hourly during a mammalian meat challenge, as described in the Methods section. CD63 expression peaked at hour 4 (63.1%) in the subject (Fig 2, A) and at hour 4 (24.5%) in a control subject (Fig 2, B). The control subject in Fig 2, C, did not show evidence of basophil activation.