RAW264.7 cells were stimulated with 100 ng/mL LPS in serum-free medium, with or without 1 µM I-BET151, for 6 or 24 h, as indicated. Supernatants were used to measure the production of IL-6 (A), TNF (B), IL-1β (C) and IL-10 (F) by ELISA (n=6; *p < 0.05 LPS treatment compared to untreated samples, **p<0.05 LPS + I-BET compared with samples treated with LPS, Mann Whitney test). RAW264.7 cells were stimulated with 100 ng/mL LPS in serum-free medium, with or without 1, 3, 6, 10, 30, or 60 µM I-BET151, for 6 h. Supernatants were used to measure the production of TNF (D) by ELISA, and cell viability was measured by the MTT assay (F) (n=3; *p < 0.05 LPS treatment compared to I-BET151-treated samples, one-way ANOVA, Bonferroni post-hoc test).