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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2014 May 22;1842(9):1549–1555. doi: 10.1016/j.bbadis.2014.05.013

Figure 2. I-BET151 does not affect phosphorylation or acetylation of p65.

Figure 2

RAW264.7 cells were stimulated with 100 ng/mL LPS in serum free medium, with or without 1 µM I-BET151, for 30 min and cell lysates (A) and nuclear extracts (B) were immunoblotted for phospho-Ser536-p65, acetyl-Lys310-p65, and total p65. β-actin (cell lysates) or CREB (nuclear extracts) were used as loading controls. Blots were quantified and graphs represent means ± SEM (n=3, *p<0.05 LPS treatment compared to untreated samples, Mann Whitney test).