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. Author manuscript; available in PMC: 2015 Jun 7.
Published in final edited form as: J Mol Microbiol Biotechnol. 2014 Jun 7;24(3):135–141. doi: 10.1159/000362722

Table 1.

Strains and oligonucleotides used in this study

Strain Relevant genotype or characteristic Source or reference
MG1655 Wild type Blattner et al (3)
ΔfruR ΔfruR in MG1655 This study
MG1655ΔlacY ΔlacY in MG1655 Klumpp et al (11)
MG1655ΔlacY ΔfruR ΔlacY ΔfruR in MG1655 This study
MG1655 (Pcrp-lacZ) MG1655ΔlacY carrying Pcrp-lacZ at the lac locus This study
MG1655 ΔfruR (Pcrp-lacZ) ΔlacY ΔfruR carrying Pcrp-lacZ at the lac locus This study
MG1655 (PcrpOCra-lacZ) ΔlacY carrying PcrpOCra-lacZ at the lac locus This study
MG1655 ΔfruR (PcrpOCra-lacZ) ΔlacY ΔfruR carrying PcrpOCra-lacZ at the lac locus This study
Oligonucleotide Sequence Use
fruR1-P1 gtgaaactggatgaaatcgctcggctggcgggagtgtcgcggaccactgcgtgtaggctggagctgcttcg fruR mutation
fruR2-P2 gctacggctgagcacgccgcggcgatagagattacgtttaatgcgcgttacatatgaatatcctccttag fruR mutation
Pcrp-Xho-F atactcgagcttgcatttttgctactccactg Cloning Pcrp into pKDT
Pcrp-Bam-R ttaggatccctggtgaataagcgtgctcttggatg Cloning Pcrp into pKDT
Pcrp-Z-P1 gcatttacgttgacaccatcgaatggcgcaaaacctttcgcggtatgtgtaggctggagctgcttc Integration of Pcrp-lacZ at the lac locus
Pcrp-Z-P2 gattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacctggtgaataagcgtgctcttggatg Integration of Pcrp-lacZ at the lac locus
Pcrp-F gttttagcatagctttcgctttgtgtctcctggtgtctaacaggagcatg Cra operator mutation in Pcrp region
Pcrp-R caccaggagacacaaagcgaaagc Cra operator mutation in Pcrp region