Table 1.
Members of the protein degradation system in the sea urchin.
| Genes (Spbase reference number) | Orthologs (organism) | % Identities | NCBI reference number | Highest signal intensity during development (AU*) | Primer sequences | Length of RNA in situ probe (nucleotid |
|---|---|---|---|---|---|---|
| Sp-Elongin B (SPU_011920) | Mm-EloB | 68.64 | NP_080581. | 4,184 | F: TCAAGCGTCAGAAGACGACC | 760 |
| Dm-EloB | 61.02 | NP_524416.1 | R: taatacgactcactatagggTGAAACGCCCCCTGGTATTC | |||
| Sp-Elongin C (SPU_001062) | Mm-EloC | 82.29 | P83940.1; NP_080732.1 | 7,673 | F: TCCTTCTAGACAACCGAACGC | 491 |
| Dm-EloC_A | 86.32 | NP_725894. | R: taatacgactcactatagggAGGCCTATAGTTTCTGACACGC | |||
| Sp-Cullin 5 (SPU_006755) | Dm-Cul5 | 72.59 | NP_651665.2 | 882 | F: AAACAATGCCCAGCTCCAGA | 783 |
| R: taatacgactcactatagggACAAAGGTTGGAGGGCACA | ||||||
| Sp-Gustavus (SPU_004717) | Dm-Gus_G | 62.5 | NP_001246140.1 | 9,277 | F: GAAAGTGTCCGGAGGCATGA | 651 |
| R: taatacgactcactatagggTCACAATGTCCCCAGACTGC | ||||||
| Sp-NeurL2 (SPU_007486) | Mm-NeurL2 | 36.84 | NP_001076443.1 | 201 | F: TGAGAACCATGGGGTGAACG | 751 |
| R: taatacgactcactatagggGCATTCCTGGAGTGTGGGAA | ||||||
| Sp-Socs4/5 (SPU_026496) | Mm-Socs5 | 47.2 | NP_062628. | 2,717 | F: TGCAAAGAACAACAGAGCCAG | 633 |
| R: taatacgactcactatagggCTTTCAGACCGTTTGGCAGC | ||||||
| Sp-Socs6L (SPU_011298) | Mm-Socs6 | 27.66 | AAH85245.1 | 2,750 | F: CGGAAAACTCGGGGGATGAA | 872 |
| R: taatacgactcactatagggGCAAGACCACCACTCTCGAA | ||||||
| Sp-Asb5 (SPU_023368) | Hs-Asb5 | 39.78 | NP_543150 | 746 | F: CAACCACCCGTACTCAGCAT | 596 |
| R: taatacgactcactatagggCCGACAGGTCAAGAGACGAG | ||||||
| Sp-Rab40 (SPU_006006) | Mm-Rab40B | 48.72 | NP_631886.2 | 1,898 | F: AGAGGAGGCCCAGGACTATG | 505 |
| R: taatacgactcactatagggTAAAACTGGATGGCGCTCTT |
*
Signal intensity as per Wei et al., 2006, an approximate measure of transcripts per embryo.