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. Author manuscript; available in PMC: 2014 Aug 7.
Published in final edited form as: Protein Expr Purif. 2010 Nov 24;76(2):229–237. doi: 10.1016/j.pep.2010.11.008

Figure 2.

Figure 2

Representative SDS-PAGEs from each of the six enzyme purifications. (M) Marker (kDa) (C) Uninduced control (WCL) Supernatant of the whole cell lysate (FT) Nickel column flow-through (W1, W2) Column wash 1 and 2 (E1, E2) Fractions of imidazole eluted protein 1 and 2. The apparent molecular weights are 37, 34, 19, 15, 19 and 67 KDa for RK, PRPPS, APRT, XGPRT, UPRT and CTPS, respectively.