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a-f. Direct and indirect continuous activity assays used for the six enzymes. (a) RK (b) PRPPS (c) APRT (d) XGPRT (e) UPRT (f) CTPS. The boxed molecule corresponds to the one being monitored during the assay at the specific wavelength with the corresponding molar extinction coefficient in M−1cm−1. MK: myokinase, PK: pyruvate kinase, LDH: lactate dehydrogenase, PEP: phopshoenolpyruvate, PPi= inorganic pyrophosphate.
