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. 2014 Jun 19;39(3):187–194. doi: 10.5395/rde.2014.39.3.187

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©Copyights 2014. The Korean Academy of Conservative Dentistry.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The effects of BMP-2 and EMD on messenger RNA expression for ALP, BSP, OCN, OPN and OSN in MC3T3-E1 cells on days 3 (a) and 7 (b). The total mRNA was extracted from the cells, and mRNA expression was determined using RT-PCR. CON, control; BMP-2, bone morphogenetic protein-2; EMD, enamel matrix derivative; MTA, mineral trioxide aggregate; ALP, alkaline phosphatase; BSP, bone sialoprotein; OCN, osteocalcin; OPN, osteopontin; OSN, osteonectin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RT-PCR, reverse-transcription polymerase chain reaction analysis.