Table 1.
Generation time after growth in minimal medium and fold expression of accA, aceF, acnA and pykF observed in L664 and of tctA mutants compared with SL1344
| Fold changea relative to SL1344 |
|||||
|---|---|---|---|---|---|
| Strain | Generation time (min) | accA | aceF | acnA | pykF |
| SL1344 | 88.78 ± 6.3 | 1 | 1 | 1 | 1 |
| L1007 (SL1344 ramR::aph) | 93.43 ± 9.4 | (1) | (1) | (1) | (1.49) |
| L664 | 128.7 ± 8.5 | 1.45 | 0.67 | 2.43 | 0.65 |
| L1316 (SL1344 glgCAP::aph) | 89.26 ± 8.2 | ND | ND | ND | ND |
| L1207 (SL1344 tctA::aph) | 131.3 ± 6.2 | 1.63 | 0.57 | 2.05 | 0.59 |
| L1269 [SL1344 tctA::aph/pGEM-tctA(WT)] | 91.28 ± 5.3 | 1.01 | 0.99 | 0.97 | 1.18 |
| L1208 [SL1344 tctA::aph/pGEM-tctA(SDM G109S)] | 122.8 ± 8.6 | 1.55 | 0.55 | 1.95 | 0.64 |
WT, wild-type; SDM, site-directed mutagenesis; ND, not determined.
Bold text indicates statistically significant differences.
aData are from RT–PCR; values in brackets are from microarray data.