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. 2014 Jul 5;13:165. doi: 10.1186/1476-4598-13-165

Figure 5.

Figure 5

HMGB1 regulated docetaxel-induced autophagy in parental LAD cells. (A) Whole cell lysates, nuclear exacts and cytoplasmic fractions from both parentalcells transfected with control or HMGB1 shRNA followed by various concentrations of docetaxel treatment for a further 48 h were subjected to western blot analysis for LC3, p62 and HMGB1. (B) SPC-A1 cells transfected with control or pcDNA3. 1-HMGB1 were treated with or without 3-MA (5 mM, 2 h) before addition of docetaxel (10 μg/l). Total cell lysates, nuclear extracts, and cytoplasmic fractions were subjected to western blot analysis for LC3, p62 and HMGB1. (C) SPC-A1 cells were co-transfected with either control or pcDNA3.1-HMGB1 and GFP-LC3 plasmid in the presence or absence of 3-MA (5 mM, 2 h). Bar = 50 μm. Values are reported as mean ± S.D. of three independent experiments. *P < 0.05, **P < 0.01.