Figure 3.

Effects of Rg1 on CCl₄-induced expression of hepatic α-SMA. Representative photomicrographs of liver histology from each group are shown as follows: Control (A), CCl₄ alone (B), CCl₄+Bicyclol (C), CCl₄+Rg1-10 mg/kg (D), CCl₄+Rg1-20 mg/kg (E) and CCl₄+Rg1-40 mg/kg (F). Original magnification: ×100. Scale bar, 100 μm. (G) Morphometrical analysis was performed for evaluating percentages of α-SMA-positive area in 12 random fields per section of six animals. (H) Western bloting analysis of α-SMA in liver tissue. Representative blots were from three independent experiments. The data were expressed as means±SD. ^cP<0.01 vs control. ^eP<0.05, ^fP<0.01 vs CCl₄ alone.