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. 2014 Jul 7;35(8):1023–1030. doi: 10.1038/aps.2014.51

Figure 1.

Figure 1

Reduced expression of SSTR2 in mESCs cultured in LIF-deprived medium. (A) Morphology of E14 mouse ES cells cultured in basal mES medium (no LIF), or media supplemented with LIF (1000 U/mL) or 2i (1 μmol/L PD0325901 and 3 μmol/L CHIR99021) for 4 d (Scale bar: 50 μm). (B) Quantitative RT-PCR analysis of pluripotency genes and SSTR2 in the E14 cells described in (A). (C) Western blot analysis of SSTR2 in mESCs cultured in basal mES medium (no LIF) or media supplemented with LIF or 2i. The data are the mean±SEM (n=3). bP<0.05, cP<0.01 vs the LIF(−) group.