Table 1. Sequences of primers used to quantify expression of genes implicated in LDLR and PCSK9 regulation.
Gene name (Human) | Accession No | Primer (5′–3′) | Product length (bp) |
---|---|---|---|
GAPDH | NM_002046.3 | AAGAAGGTGGTGAAGCAGG AGGTGGAGGAGTGGGTGTCG | 109 |
PCSK9 | NM_174936.3 | CCAAGCCTCTTCTTACTTCACC GCATCGTTCTGCCATCACT | 111 |
LDLR | NM_000527.4 | CTGAAATCGCCGTGTTACTG GCCAATCCCTTGTGACATCT | 92 |
SREBP-1c | NM_001005291.2 | GCAGGGGTAGGGCCAACGGCCT CATGTCTTCGAAAGTGCAATCC | 80 |
FAS | NM_004104.4 | TGTGGACATGGTCACGGAC GGCATCAAACCTAGACAGGTC | 100 |
SCD | NM_005063.4 | AGTCTGGCTTTGCTCTATTAAGAT CTTCCCTTAGATCCTTTTCTACTG | 192 |
SREBP-2 | NM_004599.3 | CTCTGACCAGCACCCACACT CACACCATTTACCAGCCATAAG | 118 |