Fig. 3.

Grouping of phosphopeptides into clusters with different temporal profiles. A, clustering of regulated phosphopeptides revealed four clusters of different temporal regulation patterns. B, pathway and functional enrichment analysis in IPA® showed overrepresentation of pathways related to cytoskeletal function in Cluster 2 (red) in the three most significantly enriched pathways, as well as function enrichment for posttranscriptional-modification-related proteins in Cluster 3 (green). Only pathways with more than two proteins with regulated phosphosites were considered in the enrichment analysis.