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. 2014 Jun 19;13(8):2017–2030. doi: 10.1074/mcp.M113.035709

Fig. 3.

Fig. 3.

Native MS of TH-pS19 in complex with 14-3-3γ. Native MS experiments were performed on purified TH-pS19 and 14-3-3γ and on their complexes. A, overlay of native mass spectra of the 14-3-3γ dimer (blue trace), the TH-pS19 tetramer (orange trace), and the (14-3-3γ)2:(TH)4:(14-3-3γ)2 complex (green trace) formed upon mixing of TH-pS19 tetramer with 14-3-3γ at a subunit mixing ratio of 1:3. B, native mass spectrum of the TH-pS19 tetramer mixed with 14-3-3γ at a subunit mixing ratio of 1:1. When we reduced the amount of 14-3-3γ, both the (14-3-3γ)2:(TH)4 complex and the (14-3-3γ)2:(TH)4:(14-3-3γ)2 complex were detected together with the free 14-3-3γ dimer.