Table II. Overview of the results obtained via native MS. Observed masses and related stoichiometries of the single components and TH:14-3-3γ complexes as determined via native MS. Expected masses and mass errors are also shown.
| Proteins | Stoichiometry of the protein complex | Measured mass (kDa) | Expected mass (kDa) | Error (%) |
|---|---|---|---|---|
| 14-3-3γ | (14-3-3γ)2 | 58.3 | 58.3 | 0.0 |
| TH | (TH)4 | 223.8 | 222.6 | 0.5 |
| TH-pS19 | (TH-pS19)4 | 224.1 | 222.9 | 0.5 |
| TH-pS40 | (TH-pS40)4 | 224.2 | 222.9 | 0.6 |
| TH-pS19pS40 | (TH-pS19pS40)4 | 224.3 | 223.2 | 0.5 |
| TH and 14-3-3γ | No complex detected between TH and 14-3-3γ | |||
| TH-pS19 and 14-3-3γ | (14-3-3γ)2:(TH-pS19)4:(14-3-3γ)2 | 340.8 | 339.5 | 0.4 |
| (14-3-3γ)2:(TH-pS19)4 | 282.7 | 281.2 | 0.5 | |
| TH-pS40 and 14-3-3γ | No complex detected between TH-pS40 and 14-3-3γ | |||
| TH-pS19pS40 and 14-3-3γ | (14-3-3γ)2:(TH-pS19p40)4:(14-3-3γ)2 | 341.0 | 339.8 | 0.4 |