Table I. Overview of N-terminal acetylation in the yeast N-terminomes of yNatA, yNatA-Δ, y[hNatA] and y[hNatA S37P]. Only Swiss-Prot database annotated N-termini (start = 1 or 2) of which the degree of Nt-Acetylation could be univocally calculated/determined in the respective setups analyzed and were compliant with the rules of N-terminal acetylation and iMet processing (54) were used for the overall calculation of Nt-Acetylation.
| yNatA |
yNatA-Δ |
y[hNatA] |
y[hNatA S37P] |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Full (%) | Full + partial (%) | Total | Full (%) | Full + partial (%) | Total | Full (%) | Full + partial (%) | Total | Full (%) | Full + partial (%) | Total | |
| NatA | ||||||||||||
| A- | 20.9 | 64.2 | 67 | 0.0 | 0.0 | 63 | 1.5 | 69.2 | 65 | 0.0 | 69.2 | 65 |
| C- | 0.0 | 0.0 | 1 | 0.0 | 0.0 | 2 | 0.0 | 0.0 | 4 | 0.0 | 0.0 | 2 |
| G- | 0.0 | 14.3 | 28 | 0.0 | 0.0 | 29 | 0.0 | 27.6 | 29 | 0.0 | 16.7 | 30 |
| S- | 84.0 | 99.6 | 238 | 0.0 | 5.2 | 211 | 8.8 | 97.8 | 227 | 0.8 | 96.6 | 236 |
| T- | 13.7 | 68.6 | 51 | 0.0 | 0.0 | 52 | 0.0 | 60.0 | 45 | 0.0 | 60.4 | 48 |
| V- | 0.0 | 13.0 | 46 | 0.0 | 4.7 | 43 | 0.0 | 18.6 | 43 | 0.0 | 15.2 | 46 |
| Total | 51.2 | 75.2 | 431 | 0.0 | 3.2 | 400 | 5.1 | 74.9 | 413 | 0.5 | 73.4 | 427 |
| NatD | ||||||||||||
| S- (H2A) | 100.0 | 0.0 | 1 | 100.0 | 0.0 | 1 | 100.0 | 0.0 | 1 | 100.0 | 0.0 | 1 |
| Total | 100.0 | 0.0 | 1 | 100.0 | 0.0 | 1 | 100.0 | 0.0 | 1 | 100.0 | 0.0 | 1 |
| NatB | ||||||||||||
| MD- | 98.3 | 100.0 | 59 | 98.1 | 100.0 | 52 | 98.6 | 100.0 | 72 | 98.4 | 100.0 | 61 |
| ME- | 97.1 | 100.0 | 35 | 96.7 | 100.0 | 30 | 97.1 | 100.0 | 34 | 91.4 | 100.0 | 35 |
| MN- | 82.0 | 100.0 | 50 | 75.6 | 100.0 | 45 | 75.5 | 100.0 | 53 | 74.0 | 100.0 | 50 |
| MQ- | 38.5 | 84.6 | 13 | 18.8 | 81.3 | 16 | 25.0 | 85.0 | 20 | 33.3 | 86.7 | 15 |
| Total | 87.9 | 98.7 | 157 | 81.8 | 97.9 | 143 | 83.2 | 98.3 | 179 | 83.2 | 98.8 | 161 |
| NatC | ||||||||||||
| MF- | 54.5 | 72.7 | 11 | 42.9 | 42.9 | 7 | 45.5 | 72.7 | 11 | 40.0 | 60.0 | 10 |
| MI- | 5.3 | 52.6 | 19 | 5.6 | 38.9 | 18 | 10.0 | 50.0 | 20 | 5.0 | 45.0 | 20 |
| ML- | 29.0 | 38.7 | 31 | 25.0 | 30.0 | 20 | 32.4 | 48.6 | 37 | 26.7 | 36.7 | 30 |
| MY- | 20.0 | 50.0 | 10 | 28.6 | 57.1 | 7 | 20.0 | 60.0 | 10 | 10.0 | 70.0 | 10 |
| Total | 25.4 | 49.3 | 71 | 21.2 | 38.5 | 52 | 26.9 | 53.8 | 78 | 20.0 | 47.1 | 70 |
| None | ||||||||||||
| P- | 0.0 | 0.0 | 31 | 0.0 | 0.0 | 31 | 0.0 | 0.0 | 29 | 0.0 | 0.0 | 26 |
| MP- | 0.0 | 0.0 | 2 | 0.0 | 0.0 | 1 | 0.0 | 0.0 | 3 | 0.0 | 0.0 | 1 |
| Total | 0.0 | 0.0 | 33 | 0.0 | 0.0 | 32 | 0.0 | 0.0 | 32 | 0.0 | 0.0 | 27 |
| Other | ||||||||||||
| MA- | 0.0 | 25.0 | 4 | 0.0 | 0.0 | 2 | 0.0 | 66.7 | 3 | 0.0 | 33.3 | 3 |
| MG- | 0.0 | 25.0 | 4 | 0.0 | 33.3 | 3 | 0.0 | 50.0 | 2 | 0.0 | 66.7 | 3 |
| MH- | 0.0 | 0.0 | 1 | 0.0 | 0.0 | 1 | ||||||
| MK- | 2.6 | 20.5 | 39 | 0.0 | 18.2 | 33 | 2.6 | 26.3 | 38 | 2.8 | 33.3 | 36 |
| MM- | 0.0 | 100.0 | 5 | 0.0 | 80.0 | 5 | 0.0 | 80.0 | 5 | 0.0 | 100.0 | 4 |
| MS- | 0.0 | 81.8 | 11 | 0.0 | 80.0 | 5 | 0.0 | 88.9 | 9 | 0.0 | 75.0 | 4 |
| MT- | 0.0 | 40.0 | 10 | 0.0 | 28.6 | 7 | 0.0 | 55.6 | 9 | 0.0 | 57.1 | 7 |
| MV- | 0.0 | 50.0 | 10 | 0.0 | 40.0 | 5 | 0.0 | 37.5 | 8 | 0.0 | 33.3 | 6 |
| Total | 1.2 | 39.8 | 83 | 0.0 | 31.1 | 61 | 1.3 | 44.0 | 75 | 1.6 | 44.4 | 63 |
| Grand total | 48.8 | 70.6 | 776 | 18.7 | 27.9 | 689 | 24.8 | 72.1 | 778 | 20.3 | 71.3 | 749 |