Fig. 4.

Standard procedure for manual dispensing. A A 96-hole spacer plate (a) is superimposed on top of a 96-well glass crystallization plate. The front protecting sheet and the 96-hole double-stick sheet are denoted by b1 and b2, respectively. The center of the spacer plate hole and the center of the well are denoted by c1 and c2, respectively. B The removable needle is fully inserted into (a) the spacer hole. (b) The 96-hole double-stick sheet. (c) The glass plate. C A schematic drawing of the syringe tip (e) region, where the needle tip-to-glass plate distance L is fixed at a desired value by the relation $L=d_1+d_2+d_3-l$, where $d_1$, $d_2$, and $d_3$ are the thicknesses of the 96-hole double-stick sheet (b1 + b2), the 96-hole spacer plate (a), and the fitting ring (f), respectively. $l$ is the syringe tip (e) length. The figures serve for illustrative purposes only, and are not drawn to scale