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. 2014 Aug 8;4:5989. doi: 10.1038/srep05989

Figure 2. Conditional expression of N-terminus-lacking Cyt f in mesophyll protoplasts from rice.

Figure 2

(a) Expression of Cyt f in cytosolic fraction from mesophyll protoplasts by immunoblot analysis. pTA7002 + 10 μM Dex, protoplasts with empty vector which were induced by 10 μM Dex. pTA7002-Cyt f + 10 μM ethanol, protoplasts with pTA7002-Cyt f which were incubated with equal amount of ethanol. pTA7002-Cyt f + 10 μM Dex, protoplasts with pTA7002-Cyt f which were induced by 10 μM Dex. Cytosolic proteins were prepared from the protoplasts at each time point (0, 6, 12 h) after treatment. (b) Immunoblotting of Lhcb1 for the same cytosolic fractions. Relative protein amounts of cytosolic proteins from different samples were normalized against protein loading. CCB, colloidal coomassie blue staining.