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. 2014 Aug 8;4:6000. doi: 10.1038/srep06000

Figure 2. Sulindac sulfide induced DR5 expression in SW480 cells.

Figure 2

(a) RNase protection assay. SW480 cells were treated with or without 200 μM sulindac sulfide for 24 h. Total RNA from SW480 cells was hybridized with probes, and then digested with RNase as described in the Materials and Methods. The housekeeping genes GAPDH and ribosomal protein L32 are shown as controls. (b) Northern blot analysis. SW480 cells were treated with various concentrations of sulindac sulfide for 24 h. Total RNA was probed with human DR5 cDNA. Ethidium bromide staining of 28S and 18S rRNA are shown as loading controls. (c) Western blotting for DR5. SW480 cells were treated with the indicated concentrations of sulindac sulfide for 24 h. β-actin was used as a loading control. (d) Western blotting for DR5. Cells were treated with or without 200 μM sulindac sulfide for the period indicated. β-actin was used as a loading control. −, treated with solvent DMSO.