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. 2014 Aug 8;4:6000. doi: 10.1038/srep06000

Figure 3. Sulindac sulfide induced DR5 promoter activity in SW480 cells.

Figure 3

SW480 cells were transiently transfected with reporter plasmids containing various sizes of DR5 promoters and the luciferase gene. Twenty-four hours after the transfection, cells were treated with or without 200 μM sulindac sulfide for 24 h, and cell lysates were the harvested for the luciferase assay, as described in the Materials and Methods. Relative luciferase activity is shown as raw light units (RLU) standardized with the protein concentrations. Data represent the means of triplicate experiments (bars, S.D.). −: treated with solvent DMSO. *: p < 0.05.