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. 2014 Aug 8;4:6000. doi: 10.1038/srep06000

Figure 6. Sulindac sulfide induced MZF1 expression, and MZF1 siRNA reduced the enhancement in DR5 expression by sulindac sulfide.

Figure 6

(a) Western blotting for MZF1. SW480 cells were treated with the indicated concentrations of sulindac sulfide for 24 h. β-actin was used as a loading control. (b) The luciferase assay was performed as described in Figure 5 with the indicated reporter plasmids. The MZF1 overexpression plasmid pcDNA3.1MZF1 was co-transfected with luciferase reporter plasmids as described in the Materials and Methods. The fold induction in promoter activity (pcDNA3.1MZF1 vs control vector pcDNA3.1) was shown as a bar graph. Data represent the means +/− S.D. of three determinations. *: p < 0.05 (c) Western blotting for DR5 and MZF1. SW480 cells were transfected with MZF1 siRNA or negative control siRNA at 80 nM. Twenty-four hours after the transfection, cells were treated with or without 200 μM sulindac sulfide for 24 h. β-actin was used as a loading control. −: treated with DMSO, mock: transfection was performed without any siRNA.