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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Mass Spectrom. 2014 Aug;49(8):665–673. doi: 10.1002/jms.3411

Figure 3.

Figure 3

MALDI FTICR IMS data for CA-derivatized GABA in rat brain cerebellum at 50 µm spatial resolution. A 12-µm thick section was thaw mounted on a MALDI target precoated with a mixture of 4-hydroxy-3-methoxycinnamaldehyde derivatization reagent and ferulic acid MALDI matrix. Shown are the (a) optical image of tissue following placement on the precoated target, (b) reconstructed ion image for m/z 264.1229 consistent within 0.4 ppm of the accurate mass of the [M+H]+ for GABA-CA (264.1230), (c) reconstructed ion image for a species at m/z 264.2682 (likely C18H34N based on mass accuracy;1.4 ppm) that shows localization to the white matter, and (d) overlay of ion images from (b) and (c).