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. Author manuscript; available in PMC: 2015 Aug 1.

Published in final edited form as: J Mass Spectrom. 2014 Aug;49(8):665–673. doi: 10.1002/jms.3411

LC-MS/MS analysis of CA derivatization products of GABA, a representative subset of isobaric compounds and rat brain cerebellum extract. (a) Compound structures and reaction scheme, (b) HPLC extracted-ion chromatograms (of fragmentation product of m/z 264) for derivatized standards and MS/MS spectra (m/z 264→) of the chromatographic CA-derivative peaks. Note: peaks at 12 and 13min have ~ identical spectra (c) MS³ spectra of the chromatographed CA-derivatives of GABA and dl-3-aminoisobutyric acid (m/z 264→ 209→). (d) HPLC extracted-ion chromatograms (m/z 264) for derivatized extraction blank, derivatized extract of rat brain cerebellum and GABA-CA standard (left panel), MS/MS spectra (m/z 264→) of the left-side chromatographic peaks (middle panel), and (MS³ spectra (m/z 264→ 209→)of the chromatographic peaks (right panel).

LC-MS/MS analysis of CA derivatization products of GABA, a representative subset of isobaric compounds and rat brain cerebellum extract. (a) Compound structures and reaction scheme, (b) HPLC extracted-ion chromatograms (of fragmentation product of m/z 264) for derivatized standards and MS/MS spectra (m/z 264→) of the chromatographic CA-derivative peaks. Note: peaks at 12 and 13min have ~ identical spectra (c) MS³ spectra of the chromatographed CA-derivatives of GABA and dl-3-aminoisobutyric acid (m/z 264→ 209→). (d) HPLC extracted-ion chromatograms (m/z 264) for derivatized extraction blank, derivatized extract of rat brain cerebellum and GABA-CA standard (left panel), MS/MS spectra (m/z 264→) of the left-side chromatographic peaks (middle panel), and (MS³ spectra (m/z 264→ 209→)of the chromatographic peaks (right panel).