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. 2013 Dec 13;12:125. doi: 10.1186/1475-2859-12-125

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Copyright © 2013 Hagström et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Constructs for co-expression of AseΔ11 and AseFAR in yeast. A) represents the generation of the construct Ase∆11/CUP1p-AseFAR/GAL1p-pYEXCHT by homologous recombination through co-transformation in Saccharomyces cerevisiae of the AseFAR (yellow), which is flanked with short sequences (red) homologous to the PvuII-linearized pYEXCHT already carrying Ase∆11 (blue). B) represents how the two ORFs were fused into a single ORF by S. cerevisiae homologous recombination, generating the construct Ase∆11-FAR/CUP1p-pYEXCHT. The ORFs are flanked with a short sequence (red) homologous to the XhoI-linearized pYEXCHT on one side, as well as with a short homologous sequence interspacing the two ORFs (green) for the purpose to aid fusion into a chimeric ORF.