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. 2014 Aug 8;8:223. doi: 10.3389/fncel.2014.00223

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Copyright © 2014 Borin, Fogli Iseppe, Pignatelli and Belluzzi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modulation of I_Kir by cAMP. (A–C) Effect of forskolin. Current tracings in control (A) and in the presence of 10 μM forskolin plus 100 μM IBMX (B); (C) comparison of the I/V curves recorded in control (blue dots) and forskolin (green dots); n = 12; the difference, tested with Two-Way ANOVA and post-hoc Bonferroni test, is significant for the potentials more negative than −80 mV. (D–F) Effect of 8Br-cAMP. Current tracings in control (D) and in the presence of 10 μM 8Br-cAMP (E); (F) Comparison of the I/V curves recorded in control (blue dots) and 8Br-cAMP (green dots); n = 6; the difference, tested with Two-Way ANOVA and post-hoc Bonferroni test, is significant for the potentials more negative than −80 mV. All recordings were realized in EC2 with the addition of BL1 and BL2, at 34°C.