Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Nat Methods. 2014 Mar 16;11(5):529–534. doi: 10.1038/nmeth.2889

Figure 2. Fosmid insertions are intact.

Figure 2

(a) Schematic of Mos1-based fosmids (Mosmids). Mos1 and cb-unc-119(+) selection recombineered into the backbone of a fosmid carrying a GFP-tagged gene. (b) Fluorescence microscopy of Mosmid insertions. Four different Mosmid insertions with GFP show expression from the tagged genes. (c) Comparative genome hybridization (CGH) of genomic DNA from four independent insertions of the Mosmid WRM0615dD02 containing tagged cnd-1. CGH is based on dense oligo arrays tiled against a genome of interest and labeling of sample DNA and control DNA with different fluorophores. Genomic regions that differ between sample and control will show a difference in the ratio between the two color intensities. The Mosmid with cnd-1:eGFP contained an error rendering the fusion protein non-fluorescent.