Table 1. Recombinant Mos1 transposon inserts at high frequency.

Five unc-119 animals were injected with a mix containing three miniMos elements carrying cb-unc-119 and Peft-3:GFP:H2B, Peft-3:mCherry or Peft-3:tdTomato:H2B transgenes. Three days later, rescued F1 animals were singled. A week later, plates were heat-shocked to express PEEL-1 and kill array-bearing animals, and insertions from rescued F1s screened for the presence of single (“single fluorophore”) or multiple (“multiple fluorophores”) transgenes. All 7 insertions from strain #5 mapped to independent genomic locations.

Injected P0 animals	#1	#2	#3	#4	#5	Total
Singled F1s (rescued)	24	45	40	18	29	156
Insertions from rescued F1s	5	5	1	1	6	18
Insertions from non-rescued F1s	0	1	0	0	1	2
Single fluorophore	5	6	1	1	7	20
Multiple fluorophores	0	0	0	0	0	0
Peft-3:GFP::H2B	1	1	1	0	2	5
Peft-3:mCherry	2	3	0	1	2	8
Peft-3:tdTomato::H2B	2	2	0	0	3	7