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Passive chloride transport in isolated perfused adult and neonatal proximal straight tubules (PST). PST were perfused in vitro at 20°C to inhibit active transport. Net chloride transport (JCl; top) was measured using the microtitration technique of Ramsay et al. (24). Inulin was used as a volume marker (n = 7 for both groups). PCl, chloride permeability.
