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Primers of catsper genes were designed for real time PCR reaction
| Gene | Primer sequences | Nucleotid (bp) | Tm ( o C) |
|---|---|---|---|
| Catsper1 | F: GTTGTTGGACGACTCTCTGAC | 21 | 83.7 |
| R: ACTTCTGTTGATGCTGTTCTACC | 23 | ||
| Catsper2 | F:CGAATGGGGCACATCACAC | 19 | 83.1 |
| R:CGAGAAGACAGAACTATCAAGGAC | 24 | ||
| Catsper3 | F:ACTATCCTCTTCATCTTGCTTGC | 23 | 82.8 |
| R:TTTGCTTCTCCTCCATAATCGC | 22 | ||
| Catsper4 | F:ACTATCCTCTTCATCTTGCTTGC | 23 | 82.4 |
| R:TCGGTGCCTTCATTGGTCTC | 20 | ||
| GAPDH | F:CGTTAATACCTCTTAATCCGGTT | 21 | 84.3 |
| R:CGGTAACAACGGGCATTACT | 20 |
