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. 2014 Jun 23;1(2):11–25. doi: 10.1002/reg2.12

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© 2014 The Authors. Regeneration published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Reporters for seven proteins are downregulated in the vicinity of the wound. (A)−(G′′′) Dissected epidermal whole mounts of larvae heterozygous for e22c‐Gal4, UAS‐DsRed2‐Nuc and the indicated reporter transgene were immunostained for Fasciclin III 4 h post‐wounding. For all genotypes, DsRed panels (A)−(G), (A′′)−(G′′), (A′′′)−(G′′′) show the location of epidermal nuclei while reporter panels (A′)−(G′), (A′′)−(G′′), (A′′′)−(G′′′) highlight all cells expressing the reporter. Fasciclin III staining (magenta, A′′′−G′′′) highlights epidermal cell membranes and the wound edge. Arrows indicate epidermal nuclei that have lost detectable reporter expression. Scale bar 200 μm.