Figure 3.

Two distinct temporal patterns of reporter regulation during wound healing. (A)−(H) Dissected epidermal whole mounts of larvae heterozygous for e22c‐Gal4, UAS‐DsRed2‐Nuc and the indicated reporter transgene were immunostained for Fasciclin III at the indicated times post‐wounding. Two examples are shown. In pattern 1 (A−D′, Sin3A^R2 reporter), expression is lost from wound edge cells within minutes of wounding. In pattern 2 (E−H′, Osa^R1 reporter), expression is lost between wounding and 4 h later. Scale bar 200 μm. (I) Graphical representation of reporter downregulation following wounding. Arrows indicate epidermal nuclei that have absent or reduced reporter expression. Yellow, epidermal nucleus expressing both DsRed and reporter; red, epidermal nucleus.