FIG. 4.

Regulatory effect of KME (500 μg/mL) and HDMPPA (50 μg/mL) on protein expression. (A) Western blot analysis of PPARγ, CD36, PPARα, LXRα, and ABCA1 protein expression. THP-1-derived macrophages were incubated with or without KME or HDMPPA for 24 h followed by the addition of oxLDL (100 μg/mL) for 48 h. Densitometric analysis of PPARγ (B), CD36 (C), PPARα (D), LXRα (E), and ABCA1 protein (F). Western blot was performed by densitometric analysis and normalized to β-actin levels. Percent changes of target gene expression were calculated by comparing their expression levels in cells with different treatments to that in oxLDL-treated cells. Data are expressed as mean±SD (n=3 in triplicates, P<.05). ^a–cIndicates significant difference.