Table 2.
Kinetics parameters and the limit of detection (LOD); the LOD was calculated by considering three standard deviations at a 0 ppb concentration of TNT; sensor response *1: it was measured when the injection of TNT solution was started.
| Sample | Monomer |
Sensor response *1 (R.U.) | Association rate constant ka (M s−1) | Dissociation rate constant kd (s−1) | LOD [ng/ml] (ppb) | |
|---|---|---|---|---|---|---|
| Molar ratio |
||||||
| MES | HEMA | |||||
| A | 1 | 0 | 201.0 | 8.43E+04 | 3.49E−04 | 271.3 |
| B | 1 | 1 | 356.3 | 1.26E+05 | 2.08E−04 | 77.1 |
| C | 1 | 2 | 566.0 | 1.80E+05 | 9.16E−04 | 245.8 |
| D | 1 | 5 | 431.0 | 5.04E+04 | 1.19E−03 | 37.0 |
| E | 1 | 10 | 714.1 | 1.77E+05 | 1.92E−03 | 8.4 |
| F | 1 | 100 | 529.8 | 1.37E+05 | 2.65E−03 | 12.9 |
| G | 1 | 1000 | 113.0 | 1.86E+05 | 2.02E−03 | 0.4 |
Time has elapsed 40 s from when the injection of antibody solution is completed.