Table 1.
GC-MS analysis of fatty acid composition.
| Time (h) | Treatment | SFA | MUFA | n3 PUFA | n6 PUFA | Total FA |
|---|---|---|---|---|---|---|
| 24 | Vehicle | 322.09 ± 38.38 | 170.21 ± 41.37 | 103.94 ± 9.56 | 349.80 ± 42.12 | 946.04 ± 119.47 |
| TCDD | 327.67 ± 14.47 | 197.54 ± 54.99 | 106.95 ± 10.60 | 363.20 ± 32.40 | 995.37 ± 99.90 | |
| PCB153 | 304.38 ± 35.47 | 134.35 ± 31.72 | 103.70 ± 11.67 | 331.29 ± 26.02 | 873.73 ± 80.71 | |
| MIX | 350.49 ± 14.14 | 264.58 ± 27.06*a | 114.20 ± 6.99 | 447.42 ± 29.96*ab | 1176.69 ± 73.34*a | |
| 72 | Vehicle | 311.76 ± 32.18 | 184.24 ± 33.04 | 93.26 ± 10.15 | 317.51 ± 22.72 | 906.77 ± 60.97 |
| TCDD | 353.26 ± 29.51 | 394.28 ± 63.50*a | 109.14 ± 2.92 | 432.07 ± 40.07* | 1288.74 ± 126.72*a | |
| PCB153 | 326.72 ± 45.42 | 213.53 ± 100.64 | 98.27 ± 15.94 | 326.28 ± 64.06 | 964.8 ± 191.49 | |
| MIX | 327.58 ± 22.21 | 257.33 ± 67.52 | 104.47 ± 5.89 | 394.45 ± 23.05 | 1083.84 ± 115.65 | |
| 168 | Vehicle | 297.26 ± 6.39 | 178.44 ± 24.29 | 97.07 ± 7.28 | 333.55 ± 14.33 | 906.32 ± 33.60 |
| TCDD | 384.92 ± 44.64* | 432.65 ± 75.20*a | 130.39 ± 6.76*a | 526.26 ± 46.53*a | 1474.21 ± 160.53*a | |
| PCB153 | 329.79 ± 46.76 | 184.20 ± 19.16 | 104.39 ± 8.46 | 342.23 ± 26.61 | 960.61 ± 95.75 | |
| MIX | 383.33 ± 14.70* | 272.31 ± 62.84*ab | 121.32 ± 11.21*a | 461.61 ± 41.32*a | 1238.56 ± 107.18*ab |
Hepatic fatty acids were measured by GC-MS. Peak areas were integrated using MassLynx software. The results are displayed in arbitrary units and reported as mean ± SD of 5 independent replicates. Data were analyzed by ANOVA followed by Tukey’s post hoc test: *p<0.05 vs. vehicle, ap<0.05 vs. PCB153 and bp<0.05 vs. TCDD.
FA – fatty acids; SFA – saturated fatty acids; MUFA – monounsaturated fatty acids; PUFA – polyunsaturated fatty acids. TCDD and PCB153 data are from Kopec et al. (2010b).