Table 1. Primer sequences for PCR and the sizes of PCR products.

Primer name	Sequence	Nucleotide position of ORFd	PCR product (ORF)
GP5 forward	CTC GAGa ATG TTG GGG AAA TGC TTG	13,788	615 bp (603 bp)
GP5 reverse	GCA TGCb CTA AGG ATG ACC CCA TTG TTC CGC	14,390
M forward	GCA TGCb ATG GGG TCG TCC TTA GAT GAC	14,375	537 bp (525 bp)
M reverse	AAG CTTc TTA TTT GGC ATA TTT GAC AAG CA	14,899

^aRestriction enzyme site for XhoI

^bRestriction enzyme site for SphI

^cRestriction enzyme site for HindIII

^dNucleotide positions of ORF5 and ORF6 in the PRRSV LMY strain