Figure 2.
Oxygen dependence of deschloro induced cellular DNA breaks by Comet assay. 2A. Representative alkaline Comet images of HCT116 cells incubated with deschloro for 1 hour at 37°C under normoxic or hypoxic (0.5% oxygen) conditions. 2B and 2C. Quantitation of at least 3 Comets tail experiments where tail intensity reflects the dose-dependent extent of DNA breaks induced by deschloro, C-1027 or NCS under normoxic (2B) and hypoxic conditions (2C).