Table 1.

Sense (S) and antisense (AS) primer sequences used for qRT-PCR of GDPD1-5

Gene	Cell lines	Tumor samples from patients
GDPD1-S	5’-GAGCTTCTGTGGGAGTACGG-3’	5’-AAGCAGCGATTCCTCAGTA-3’
GDPD1-AS	5’-CGCAGGAAGAAGATGGAGAG-3’	5’-AGTCCAATTCTAGCATATCAGTTC-3’
GDPD2-S	5’-TGCTGCTGACAAGGATCAAC-3’	5’-ACTGTGTTTGAGACTGAT-3’
GDPD2-AS	5’-CCCCTGAAGCATTCCACTTA-3’	5’-GGAATACAGAGGCTACAT-3’
GDPD3-S	5’-CCACACACCATGTCCAGAAG-3’	5’-TTCGTGAGACGCTATGAC-3’
GDPD3-AS	5’-GAATGCCTCGAGCAGTTAGG-3’	5’-ATCCTCGGCTTATTGTGAA-3’
GDPD4-S	5’-CCCAAGAGTGGAAAGGAACA-3’	5’-TCAGCATAACTGTGATGGT-3’
GDPD4-AS	5’-GCTTCAAAGGTGTGACAGCA-3’	5’-CACTTGTAGACCTCTTAACCT-3’
GDPD5-S	5’-AGCAGTCACCATGCTCTCCT-3’	5’-CTACAACCCTGAGCAGAT-3’
GDPD5-AS	5’-AAACACCACGGTGAAGAAGG-3’	5’-AACATACGGAGAGCACAT-3’
CHKA-S	5’-GATCCGAACAAGCTCAGAAAGAAAATG-3’	5’-GATCCGAACAAGCTCAGAAAGAAAATG-3’
CHKA-AS	5’-CGGCTCGGGATGAACTGCTC-3’	5’-CGGCTCGGGATGAACTGCTC-3’
PLD1-S	-	5’-ATGGAATCCGAATTGATAATCTT-3’
PLD1-AS		5’-AGCATTCTCTTGGATAGCA-3’
Housekeeping genes
36B4-S	5’-GATTGGCTACCCAACTGTTGCA-3’
36B4-AS	5’-CAGGGGCAGCAGCCACAAAGGC-3’
HPRT1-S	5’-CCTGGCGTCGTGATTAGTGATG-3’
HPRT1-AS	5’-CAGAGGGCTACAATGTGATGGC-3’

All GDPD primers were designed to be gene specific and not transcript specific.