Skip to main content
. Author manuscript; available in PMC: 2015 Jul 14.
Published in final edited form as: Cancer Cell. 2014 Jul 14;26(1):48–60. doi: 10.1016/j.ccr.2014.05.001

Figure 1.

Figure 1

CRL4DCAF1 controls YAP activation and TEAD-dependent transcription in Merlin-deficient cells. (A) Schematics depict wild type (WT) and Merlin-resistant (1417X) DCAF1 (top). Meso-33 cells were transfected with HA-Merlin alone or in combination with HA-DCAF1 WT or 1417X and subjected to immunoblotting. (B) Meso-33 cells were transfected with the indicated si-RNAs and examined by immunoblotting. (C) Nf2 mutant FC-1801 cells were transduced with the indicated sh-RNAs and subjected to immunofluorescent staining with anti-YAP/TAZ. Nuclei were stained with DAPI. Scale bar, 10μm. (D) FC-1801 cells were transduced with either a control sh-RNA, a sh-RNA targeting DCAF1, Merlin, or dominant negative TEAD2 followed by a TEAD reporter, and subjected to luciferase assay. Error bar, +/−SEM. (E) and (G) Meso-33 cells were transfected with the indicated si-RNAs and immunoblotted as indicated. (F) and (H) Meso-33 cells were transfected with the indicated si-RNAs and then with empty vector or Merlin and immunoblotted as indicated. See also Figure S1.