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. Author manuscript; available in PMC: 2015 Jul 14.
Published in final edited form as: Cancer Cell. 2014 Jul 14;26(1):48–60. doi: 10.1016/j.ccr.2014.05.001

Figure 6.

Figure 6

CRL4DCAF1-mediated inhibition of Lats and de-regulated YAP signaling sustain the oncogenic properties of Merlin-deficient tumor cells. (A) Meso-33 cells expressing GFP, YAP, or YAP-5SA were transfected with a SMART pool control si-RNA or one targeting DCAF1 and subjected to BrdU incorporation assay. (B) FC-1801 cells expressing empty vector or YAP-5SA were transduced with the indicated sh-RNAs and subjected to soft agar assay. The graph illustrates the results (±SEM) normalized to relative control in the vector or YAP-5SA group. (C) FC-1801 cells treated as in (B) were injected subcutaneously in nude mice. (D) Meso-33 cells transduced with a control sh-RNA or sh-RNAs targeting Lats1/2 were transduced with a control sh-RNA or two different sh-RNAs targeting DCAF1 and subjected to BrdU incorporation assay. (E) Meso-33 cells treated as in (D) were subjected to soft agar assay. Typical cell colonies in individual culture wells in the 24-well plate are shown (F). (G) FC-1801 cells transduced with a control sh-RNA (top) or sh-RNAs targeting Lats1/2 (bottom) were subsequently transduced with a control sh-RNA or a sh-RNA targeting DCAF1 and were injected subcutaneously in nude mice. Error bar, +/− SEM. See also Figure S6.