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. 2014 Apr 22;3:93. [Version 1] doi: 10.12688/f1000research.3720.1

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Copyright: © 2014 Fameli N et al.

This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).

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Figure 3. — A, High magnification (150,000×) electron micrograph of a region of Figure 2B containing 3 L-SR junctions (arrows); coloured tracings as shown were used to measure lysosome dimensions, L-SR widths and extensions. Scale bar: 100 nm. B– D, Histograms showing distribution of several relevant lysosomal and L-SR junctional parameters, used to characterize the junctions and inform the quantitative model. B, lysosomal dimensions as major and minor axes of oval shapes in micrographs; C, L-SR junctional width; D, percentage apposition between junctional SR and lysosome perimeter as projected in 2D micrographs.