Table 2. Quantitative model input data.

Quantity	Value	Source
Δ[Ca 2+] shoulder	300 nM	[ 17] and this article
[Ca 2+] lys	400–600 μM	[ 44]
lysosomal average radius	180 nm	this article, Figure 3
(cylindrical) SMC radius	6 μm	[ 46]
SMC length	130 μm	[ 46]
SMC cytosolic volume	2.4×10 −12 L	see Supplementary material
TPC2 complexes per lysosome	20	[ 48, 45] and this article
TPC2 complex Ca 2+ release rate	variable	this article, Figure 5
SERCA2a surface density	6250/ μm2	[ 39]
SERCA2a first Ca 2+ binding (unbinding) rate	5×10 8M −1s −1 (60 s −1)	[ 50– 52]
SERCA2a second Ca 2+ binding (unbinding) rate	4×10 8M −1s −1 (60 s −1)	[ 50– 52]
free Ca 2+ diffusivity, D free	2.23×10 −10 m 2/s	[ 53, 54]
Ca 2+ buffer diffusivity, D buffer	8.4×10 −11 m 2/s	[ 55]

Summary of input parameters used in various phases of the quantitative model, and references to the origin of their values.