Table 1.
Strains used and constructed in this study.
| Strain | Description | Reference |
|---|---|---|
| Escherichia coli DH5α | E. coli strain used for cloning and plasmid construction | New England Biolabs |
| Synechococcus elongatus PCC 7942 | Model freshwater cyanobacterium (ATCC 33912); previously used as host for FFA biosynthesis (Ruffing and Jones, 2012; Ruffing, 2013a,b) | American type culture collection |
| Synechococcus sp. PCC 7002 | Model marine cyanobacterium (ATCC 27264); host for FFA biosynthesis | American type culture collection |
| SE01 | S. elongatus PCC 7942 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (aas) | Ruffing and Jones (2012) |
| SE02 | S. elongatus PCC 7942 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (aas) and expression of truncated E. coli thioesterase (‘tesA) | Ruffing and Jones (2012) |
| SE03 | S. elongatus PCC 7942 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (aas) and expression of C. reinhardtii acyl-ACP thioesterase (fat1) | Ruffing (2013a) |
| SE04 | S. elongatus PCC 7942 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (aas), expression of C. reinhardtii acyl-ACP thioesterase (fat1), and expression of rbcLS (Ptrc-fat1-rbcLS) | Ruffing (2013a) |
| SE06 | S. elongatus PCC 7942 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (aas), expression of C. reinhardtii acyl-ACP thioesterase (fat1), and expression of RuBisCO (rbcLS) from the psbAI promoter of S. elongatus PCC 7942 (Ptrc-fat1-PpsbAI-rbcLS) | Ruffing (2013a) |
| S01 | Synechococcus sp. PCC 7002 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (fadD) | This study |
| S02 | Synechococcus sp. PCC 7002 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (fadD) and expression of truncated E. coli thioesterase (‘tesA) | This study |
| S02ΔdesB | Synechococcus sp. PCC 7002 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (fadD) and desaturase B (desB), along with expression of truncated E. coli thioesterase (‘tesA) | This study |
| S03 | Synechococcus sp. PCC 7002 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (fadD) and expression of C. reinhardtii acyl-ACP thioesterase (fat1) | This study |
| S05 | Synechococcus sp. PCC 7002 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (fadD) and expression of a truncated C. reinhardtii acyl-ACP thioesterase (tfat1) | This study |
| S06 | Synechococcus sp. PCC 7002 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (fadD), expression of truncated E. coli thioesterase (‘tesA), and expression of RuBisCO (rbcLS) (Ptrc-‘tesA-rbcLS) | This study |
| S07 | Synechococcus sp. PCC 7002 with gene knockout of the acyl-ACP synthetase/long-chain-fatty-acid CoA ligase (fadD), expression of truncated E. coli thioesterase (‘tesA), and expression of RuBisCO (rbcLS) from the psbAI promoter of S. elongatus PCC 7942 (Ptrc-‘tesA-PpsbAI-rbcLS) | This study |