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. 2014 Aug 8;9(8):e103774. doi: 10.1371/journal.pone.0103774

Figure 4. MALT1 p76 activates NF-κB signalling in 293T cells.

Figure 4

A) Features of Flag-tagged MALT1, p76, MALT1-C and MALT1-p19. Numbers indicate the start and stop AA position for p76, MALT1-C and MALT1-p19 relative to the MALT1 protein sequence (Refseq NP_006776.1, 824 AA). B-C) NF-κB-reporter assays of 293T cells transiently expressing MALT1, mp-MALT1, MALT1-p19, p76 and mutants or MALT1-C. NF-κB-dependent luciferase activity is shown as fold induction of vector-transfected cells and represents the mean +/- S.D. (n = 3). Immunoblot of cell lysates with a-Flag – a-β-actin shows equal expression/loading of the different MALT1 constructs (B) and a-MALT1-C (C) shows equal expression of the different MALT1 constructs. Bottom (C): streptavidin pull-down (bio-IP) of MALT1, p76 or MALT1-C, transiently expressed in 293T cells, and immunoblotted with a-MALT1C, a-TRAF6 and a-BCL10 antibodies. e-MALT1, e-TRAF6, e-BCL10: endogenous MALT1, TRAF6 and BCL10. D) immunoblot of bio-IPs of Avi-tagged Ub-p76 expressed together with Flag-p76 or Flag MALT1 with a-MALT1-C, a-Flag and a-TRAF6. * indicate non-specific bands.