Table 1.
Derivatives with ortho-substituent groups.
| |||||
|---|---|---|---|---|---|
| Compound | IC50[a] (nM) | MIC[b] (μg/mL) | Ki (nM) | Slow-onset | Residence Time (min) |
| R | |||||
| PT004 -H | 11 ± 1[c] | 2.1 | 9.4 ± 0.5 | No | |
| PT010 -NO2 | 182 ± 2.0[d] | 12.50 | N.D.[f] | Yes | 27 ± 6 |
| PT013 -NH2 | 61.9 ±4.5[d] | 3.13 | N.D.[f] | No | |
| PT070 -CH3 | 50.7 ± 4[d] | 3.125 | 0.044 ± 0.005 | Yes | 24 ± 2 |
| PT091 -Cl | 49.5 ± 2.2[d] | 1.56 | 0.96 ± 0.14 | Yes | 21 ± 3 |
| PT092 -Br | 10.0 ± 0.8[e] | 3.125 | 0.20 ± 0.05 | Yes | 30 ± 3 |
| PT095 -CF3 | 29.7 ± 1.2[e] | 50.00 | N.D.[f] | No | |
| PT096 -I | 44.6 ± 7.5[d] | 25 | 3.72 ± 5.13 | No | |
| PT113 -F | 12.1 ± 4.8[e] | 1.56 | 0.09 ± 0.02 | Yes | 9 ± 3 |
| PT114 -OH | 48 ± 3[d] | 12.5 | 15.9 ± 3.7 | No | |
| PT119 -CN | 235.6 ± 10.0[d] | 2.5 | 2.14 ± 0.35 | Yes | 80 ± 12 |
[a]
The half maximal inhibitory concentration (IC50) is the concentration of an inhibitor that is required for inhibition of 50% of the enzyme activity.
[b]
The minimum inhibitory concentration (MIC) is the lowest concentration of an inhibitor that is adequate to inhibit visible growth of bacteria.
[c]
IC50 values were determined at an enzyme concentration of 1 nM.
[d]
IC50 values were determined at an enzyme concentration of 100 nM.
[e]
IC50 values were determined at an enzyme concentration of 20 nM.
[f]
N.D. = Not determined.
Values of IC50, Ki, and residence time are average values from 3 independent experiments with standard deviation.