Table 3.
Derivatives with heterocyclic B-rings.
Compound | IC50 (nM)[a][c] | MIC[b] (μg/mL) | Slow-onset | Residence time (min) | ||
---|---|---|---|---|---|---|
R | ||||||
PT077 |
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H | 190 ± 12 | 3.13 | No | |
PT164 | -CN | 308 ± 4.2 | <0.39 | Yes | N.D.[d] | |
PT161 | -F | 40 ± 20 | 0.3125 | Yes | N.D.[d] | |
PT112 |
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-NH2 | 69000 ± 700 | 50 | N.D.[d] | |
PT115 | -CN | 890.6 ± 105.8 | 12.5 | Yes | >20 | |
PT116 | -COOH | >12000 | 25 | N.D.[d] | ||
PT134 |
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238.8 ± 46.8 | 3.125 | N.D.[d] |
The half maximal inhibitory concentration (IC50) is the concentration of an inhibitor that is required for inhibition of 50% of the enzyme activity.
The minimum inhibitory concentration (MIC) is the lowest concentration of an inhibitor that is adequate to inhibit visible growth of bacteria.
IC50 values were determined at an enzyme concentration of 100 nM.
N.D. = Not determined.
IC50 and Ki values are average results from 3 independent experiments with standard deviation.